Bacterial and fungal profile of diabetic foot ulcer (2023)

Devasia, A., John, L. A., Velladath, S. U., Prakash, P. Y., Mohammad, C. A. (2022). Bacterial and fungal profile of diabetic foot ulcer. Biomedicine (India), 42(6), 1372-1375. https://doi.org/10.51248/.V42I6.2438

Devasia, Anjally ; John, Linu Anice ; Velladath, Saleena Ummer et al. / Bacterial and fungal profile of diabetic foot ulcer. In: Biomedicine (India). 2022 ; Vol. 42, No. 6. pp. 1372-1375.

@article{06a9e34c2c6a4d44924f6638b84c9a2e,

title = "Bacterial and fungal profile of diabetic foot ulcer",

abstract = "Introduction: Polymicrobial biofilm in chronic diabetic foot wounds is a major challenge for the treatment and lead to several complications, including increased morbidity, mortality, and limb loss. Methodology: In this study, a spectrum of bacterial and fungal isolates was carried out in 66-foot ulcer samples from diabetic patients. All bacterial isolates were identified by conventional culture methods and biochemical identification tests. Filamentous fungi and yeasts were identified morphologically and by staining methods. Results: Among 66 samples, 62 (94%) samples were culture-positive, and four samples (6%) were culture-negative by the conventional culture method. Polymicrobial growth was found in 11 samples (17%) and monomicrobial in 51 wound samples (77%). Both Gram-positive and Gram-negative bacterial species were isolated. The number of Staphylococcus aureus isolates was more (61%). Fungi were isolated in 4 patients, and all the isolates were identified as Candida tropicalis. Several clinical complications were found including hypertension (47%), osteoarthritis (6%), rheumatoid arthritis (8%), cardiovascular disease (19%), peripheral vascular disease (20%), amputation (35%), foot cramps (71%) and edema (8%). Conclusion: Monomicrobial growth was predominant than polymicrobial growth, and S. aureus was the predominant pathogen isolated. Adequate treatment management of diabetic foot is needed to reduce the risk of amputation.",

author = "Anjally Devasia and John, {Linu Anice} and Velladath, {Saleena Ummer} and Prakash, {Peralam Yegneswaran} and Mohammad, {Ciraj Ali} and Shettigar, {Kavitha S.}",

note = "Funding Information: The authors thank Manipal Academy of Higher Education for providing the infrastructure & financial support. Publisher Copyright: {\textcopyright} 2022, Indian Association of Biomedical Scientists. All rights reserved.",

year = "2022",

doi = "10.51248/.V42I6.2438",

language = "English",

volume = "42",

pages = "1372--1375",

journal = "Biomedicine",

issn = "0970-2067",

publisher = "Indian Association of Biomedical Scientists",

number = "6",

}

Devasia, A, John, LA, Velladath, SU, Prakash, PY, Mohammad, CA 2022, 'Bacterial and fungal profile of diabetic foot ulcer', Biomedicine (India), vol. 42, no. 6, pp. 1372-1375. https://doi.org/10.51248/.V42I6.2438

Bacterial and fungal profile of diabetic foot ulcer. / Devasia, Anjally; John, Linu Anice; Velladath, Saleena Ummer et al.

In: Biomedicine (India), Vol. 42, No. 6, 2022, p. 1372-1375.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Bacterial and fungal profile of diabetic foot ulcer

AU - Devasia, Anjally

AU - John, Linu Anice

AU - Velladath, Saleena Ummer

AU - Prakash, Peralam Yegneswaran

AU - Mohammad, Ciraj Ali

AU - Shettigar, Kavitha S.

N1 - Funding Information:The authors thank Manipal Academy of Higher Education for providing the infrastructure & financial support.Publisher Copyright:© 2022, Indian Association of Biomedical Scientists. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Introduction: Polymicrobial biofilm in chronic diabetic foot wounds is a major challenge for the treatment and lead to several complications, including increased morbidity, mortality, and limb loss. Methodology: In this study, a spectrum of bacterial and fungal isolates was carried out in 66-foot ulcer samples from diabetic patients. All bacterial isolates were identified by conventional culture methods and biochemical identification tests. Filamentous fungi and yeasts were identified morphologically and by staining methods. Results: Among 66 samples, 62 (94%) samples were culture-positive, and four samples (6%) were culture-negative by the conventional culture method. Polymicrobial growth was found in 11 samples (17%) and monomicrobial in 51 wound samples (77%). Both Gram-positive and Gram-negative bacterial species were isolated. The number of Staphylococcus aureus isolates was more (61%). Fungi were isolated in 4 patients, and all the isolates were identified as Candida tropicalis. Several clinical complications were found including hypertension (47%), osteoarthritis (6%), rheumatoid arthritis (8%), cardiovascular disease (19%), peripheral vascular disease (20%), amputation (35%), foot cramps (71%) and edema (8%). Conclusion: Monomicrobial growth was predominant than polymicrobial growth, and S. aureus was the predominant pathogen isolated. Adequate treatment management of diabetic foot is needed to reduce the risk of amputation.

AB - Introduction: Polymicrobial biofilm in chronic diabetic foot wounds is a major challenge for the treatment and lead to several complications, including increased morbidity, mortality, and limb loss. Methodology: In this study, a spectrum of bacterial and fungal isolates was carried out in 66-foot ulcer samples from diabetic patients. All bacterial isolates were identified by conventional culture methods and biochemical identification tests. Filamentous fungi and yeasts were identified morphologically and by staining methods. Results: Among 66 samples, 62 (94%) samples were culture-positive, and four samples (6%) were culture-negative by the conventional culture method. Polymicrobial growth was found in 11 samples (17%) and monomicrobial in 51 wound samples (77%). Both Gram-positive and Gram-negative bacterial species were isolated. The number of Staphylococcus aureus isolates was more (61%). Fungi were isolated in 4 patients, and all the isolates were identified as Candida tropicalis. Several clinical complications were found including hypertension (47%), osteoarthritis (6%), rheumatoid arthritis (8%), cardiovascular disease (19%), peripheral vascular disease (20%), amputation (35%), foot cramps (71%) and edema (8%). Conclusion: Monomicrobial growth was predominant than polymicrobial growth, and S. aureus was the predominant pathogen isolated. Adequate treatment management of diabetic foot is needed to reduce the risk of amputation.

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DO - 10.51248/.V42I6.2438

M3 - Article

AN - SCOPUS:85146652837

VL - 42

SP - 1372

EP - 1375

JO - Biomedicine

JF - Biomedicine

SN - 0970-2067

IS - 6

ER -

Devasia A, John LA, Velladath SU, Prakash PY, Mohammad CA, Shettigar KS. Bacterial and fungal profile of diabetic foot ulcer. Biomedicine (India). 2022;42(6):1372-1375. doi: 10.51248/.V42I6.2438